The figure down below exhibits the calibration curve and calibration equation for that set of exterior standards. Substituting the sample’s peak spot into your calibration equation presents the concentration of caffeine inside the sample as 94.four mg/L.Exactly where N is the amount of theoretical plates, L is definitely the length in the column,
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Slim-bore columns (1–2 mm) are used for applications when much more sensitivity is wished-for either with Unique UV-vis detectors, fluorescence detection or with other detection techniques like liquid chromatography-mass spectrometryHPLC is usually a broad analytical chemistry system utilized to individual, identify and quantify compounds inside